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human hdl  (Lee Biosolutions)


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    Structured Review

    Lee Biosolutions human hdl
    Human Hdl, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hdl/Human+Plasma+High+Density+Lipoprotein+-+Sterile+Filtered/pmc12627944-141-16-18
    Average 92 stars, based on 6 article reviews
    human hdl - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Angiopoietin-like 3 inhibition of endothelial lipase is not modulated by angiopoietin-like 8
    Article Snippet: .. EL (final concentration of ∼60 μg/ml) and ANGPTL proteins (0–2,600 ng/ml ANGPTL3; 0–78 ng/ml ANGPTL8) were incubated together at 37°C for 30 min. Human HDL (Lee Biosolutions) was added to the sample to a final concentration of 62 mg/dl (cholesterol) and incubated at 37°C for an additional 30 min. After incubation, 50 μl of sample was loaded into a Greiner clear 96-well plate. .. NEFA levels in each sample were measured using a commercial kit (HR series NEFA-HR; Wako) and following the manufacturer's instructions.

    Article Title: Compromised lipid metabolism, mitochondria respiration and neuroprotective effects in iPSC-derived astrocytes from a Smith-Lemli-Opitz syndrome patient
    Article Snippet: NPCs were either cryopreserved in StemMACS cryo-brew (Miltenyi Biotec, 130–109-558) or expanded in neural precursor media containing DMEM, B-27 without vitamin A, bFGF (20 ng/ml), EGF (20 ng/ml), glutamine (2 mM), and penicillin–streptomycin (50 μg/ml). .. To allow SLOS NPC formation and proliferation [ ], sterile-filtered human LDL (Lee Biosolutions, 360–12) and human HDL (Lee Biosolutions, 361–12) were each added to a final concentration of 15 μg/ml. .. NPCs also received 1:400 dilution of chemically defined lipid concentrate (Thermo Fisher Scientific, 11 905 031).

    Article Title: Compromised lipid metabolism, mitochondria respiration and neuroprotective effects in iPSC-derived astrocytes from a Smith-Lemli-Opitz syndrome patient.
    Article Snippet: Smith-Lemli-Opitz syndrome (SLOS) is a rare, autosomal recessive disorder characterized by congenital malformations, intellectual disability, and behavioral abnormalities.. SLOS results from mutations in the DHCR7 gene, leading to impaired cholesterol biosynthesis due to dysregulation of 7-dehydrocholesterol reductase.. Cholesterol plays crucial roles in neurophysiology, including synaptic formation and neurotransmitter receptor regulation, which likely contribute to neurological manifestations in SLOS patients.

    Incubation:

    Article Title: Angiopoietin-like 3 inhibition of endothelial lipase is not modulated by angiopoietin-like 8
    Article Snippet: .. EL (final concentration of ∼60 μg/ml) and ANGPTL proteins (0–2,600 ng/ml ANGPTL3; 0–78 ng/ml ANGPTL8) were incubated together at 37°C for 30 min. Human HDL (Lee Biosolutions) was added to the sample to a final concentration of 62 mg/dl (cholesterol) and incubated at 37°C for an additional 30 min. After incubation, 50 μl of sample was loaded into a Greiner clear 96-well plate. .. NEFA levels in each sample were measured using a commercial kit (HR series NEFA-HR; Wako) and following the manufacturer's instructions.

    Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis
    Article Snippet: .. Cells were labeled with 0.5 μCi ml −1 of H-cholesterol (PerkinElmer) and 37.5 μg ml −1 acetylated LDL (Kalen Biomedical, LLC 770201-6) for 24 h, washed twice with PBS and then incubated with 2 mg ml −1 fatty-acid-free BSA (Sigma-Aldrich) in medium for 24 h. To induce efflux, we added 50 μg ml −1 human HDL (Lee biosolutions 361-10-0.1) in fatty-acid-free medium, and supernatants were collected after 24 h. HDL-dependent efflux was expressed as a percentage of total cell H-cholesterol content. ..

    Article Title: Endothelial Lipase Variant, T111I, Does Not Alter Inhibition by Angiopoietin-like Proteins
    Article Snippet: .. EL conditioned media and 67 or 116.4 mg/dL (cholesterol) of human HDL (Lee Biosolutions) were incubated together for 60 minutes at 37°C. .. After incubation, 50 μL of sample was loaded into a Greiner clear 96-well plate.

    Labeling:

    Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis
    Article Snippet: .. Cells were labeled with 0.5 μCi ml −1 of H-cholesterol (PerkinElmer) and 37.5 μg ml −1 acetylated LDL (Kalen Biomedical, LLC 770201-6) for 24 h, washed twice with PBS and then incubated with 2 mg ml −1 fatty-acid-free BSA (Sigma-Aldrich) in medium for 24 h. To induce efflux, we added 50 μg ml −1 human HDL (Lee biosolutions 361-10-0.1) in fatty-acid-free medium, and supernatants were collected after 24 h. HDL-dependent efflux was expressed as a percentage of total cell H-cholesterol content. ..

    Sterility:

    Article Title: Compromised lipid metabolism, mitochondria respiration and neuroprotective effects in iPSC-derived astrocytes from a Smith-Lemli-Opitz syndrome patient
    Article Snippet: NPCs were either cryopreserved in StemMACS cryo-brew (Miltenyi Biotec, 130–109-558) or expanded in neural precursor media containing DMEM, B-27 without vitamin A, bFGF (20 ng/ml), EGF (20 ng/ml), glutamine (2 mM), and penicillin–streptomycin (50 μg/ml). .. To allow SLOS NPC formation and proliferation [ ], sterile-filtered human LDL (Lee Biosolutions, 360–12) and human HDL (Lee Biosolutions, 361–12) were each added to a final concentration of 15 μg/ml. .. NPCs also received 1:400 dilution of chemically defined lipid concentrate (Thermo Fisher Scientific, 11 905 031).

    Article Title: Compromised lipid metabolism, mitochondria respiration and neuroprotective effects in iPSC-derived astrocytes from a Smith-Lemli-Opitz syndrome patient.
    Article Snippet: Smith-Lemli-Opitz syndrome (SLOS) is a rare, autosomal recessive disorder characterized by congenital malformations, intellectual disability, and behavioral abnormalities.. SLOS results from mutations in the DHCR7 gene, leading to impaired cholesterol biosynthesis due to dysregulation of 7-dehydrocholesterol reductase.. Cholesterol plays crucial roles in neurophysiology, including synaptic formation and neurotransmitter receptor regulation, which likely contribute to neurological manifestations in SLOS patients.



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    Lee Biosolutions high density lipoproteins
    (A) PACERR expression in total RNA isolated from human carotid endarterectomy samples where advanced/central plaques are separated from peripheral plaque and sequencing was done on both. (B) scRNA-seq of carotid plaque samples. (C) THP-1 cells differentiated into macrophages using 10 nM PMA for 48–72 h then treated with ApoA1 (50µg/ml), HDL (50µg/ml), acLDL (37.5µg/ml), oxLDL (10µg/ml), or LPS (100ng/ml) for 24 hours followed by qPCR for gene expression analysis. (D) THP-1 macrophages were treated with 50µg/ml HDL, <t>HDL2</t> or <t>HDL3</t> for 24 hours and qPCR for gene expression was used, western blot to measure COX-2 protein levels and mass spectrometry was used to measure 6k-PGF1a/PGI2 and PGE2 in the cell media. (E) THP-1 macrophages were pre-treated with 5µM celecoxib for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (F) THP-1 macrophages were pre-treated with 5µM Bay-117082 for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (G) THP-1 macrophages stably expressing CREB shRNA were treated with 50 µg/ml or 100ng/ml LPS for 24 hours then qPCR was used to measure gene expression. Values are mean ±SEM of three independent experiments. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 versus untreated.
    High Density Lipoproteins, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lee Biosolutions human hdls
    (A) PACERR expression in total RNA isolated from human carotid endarterectomy samples where advanced/central plaques are separated from peripheral plaque and sequencing was done on both. (B) scRNA-seq of carotid plaque samples. (C) THP-1 cells differentiated into macrophages using 10 nM PMA for 48–72 h then treated with ApoA1 (50µg/ml), HDL (50µg/ml), acLDL (37.5µg/ml), oxLDL (10µg/ml), or LPS (100ng/ml) for 24 hours followed by qPCR for gene expression analysis. (D) THP-1 macrophages were treated with 50µg/ml HDL, <t>HDL2</t> or <t>HDL3</t> for 24 hours and qPCR for gene expression was used, western blot to measure COX-2 protein levels and mass spectrometry was used to measure 6k-PGF1a/PGI2 and PGE2 in the cell media. (E) THP-1 macrophages were pre-treated with 5µM celecoxib for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (F) THP-1 macrophages were pre-treated with 5µM Bay-117082 for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (G) THP-1 macrophages stably expressing CREB shRNA were treated with 50 µg/ml or 100ng/ml LPS for 24 hours then qPCR was used to measure gene expression. Values are mean ±SEM of three independent experiments. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 versus untreated.
    Human Hdls, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) PACERR expression in total RNA isolated from human carotid endarterectomy samples where advanced/central plaques are separated from peripheral plaque and sequencing was done on both. (B) scRNA-seq of carotid plaque samples. (C) THP-1 cells differentiated into macrophages using 10 nM PMA for 48–72 h then treated with ApoA1 (50µg/ml), HDL (50µg/ml), acLDL (37.5µg/ml), oxLDL (10µg/ml), or LPS (100ng/ml) for 24 hours followed by qPCR for gene expression analysis. (D) THP-1 macrophages were treated with 50µg/ml HDL, HDL2 or HDL3 for 24 hours and qPCR for gene expression was used, western blot to measure COX-2 protein levels and mass spectrometry was used to measure 6k-PGF1a/PGI2 and PGE2 in the cell media. (E) THP-1 macrophages were pre-treated with 5µM celecoxib for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (F) THP-1 macrophages were pre-treated with 5µM Bay-117082 for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (G) THP-1 macrophages stably expressing CREB shRNA were treated with 50 µg/ml or 100ng/ml LPS for 24 hours then qPCR was used to measure gene expression. Values are mean ±SEM of three independent experiments. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 versus untreated.

    Journal: bioRxiv

    Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis

    doi: 10.1101/2025.06.02.657432

    Figure Lengend Snippet: (A) PACERR expression in total RNA isolated from human carotid endarterectomy samples where advanced/central plaques are separated from peripheral plaque and sequencing was done on both. (B) scRNA-seq of carotid plaque samples. (C) THP-1 cells differentiated into macrophages using 10 nM PMA for 48–72 h then treated with ApoA1 (50µg/ml), HDL (50µg/ml), acLDL (37.5µg/ml), oxLDL (10µg/ml), or LPS (100ng/ml) for 24 hours followed by qPCR for gene expression analysis. (D) THP-1 macrophages were treated with 50µg/ml HDL, HDL2 or HDL3 for 24 hours and qPCR for gene expression was used, western blot to measure COX-2 protein levels and mass spectrometry was used to measure 6k-PGF1a/PGI2 and PGE2 in the cell media. (E) THP-1 macrophages were pre-treated with 5µM celecoxib for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (F) THP-1 macrophages were pre-treated with 5µM Bay-117082 for 1 hour before addition of 50µg/ml HDL or 100ng/ml LPS for 24 hours. (G) THP-1 macrophages stably expressing CREB shRNA were treated with 50 µg/ml or 100ng/ml LPS for 24 hours then qPCR was used to measure gene expression. Values are mean ±SEM of three independent experiments. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 versus untreated.

    Article Snippet: High density lipoproteins (HDL, HDL2 and HDL3) were purchased from Lee biosolutions (cat # 361-10-0.1) or academy biomedical solutions (80P-HD-101, 80P-HD2-101, 80P-HD3-101) and used at 50µg/ml, acetylated LDL (acLDL) was purchased from Kalen Biomedical, LLC (770201-6) and used at 37.5µg/ml.

    Techniques: Expressing, Isolation, Sequencing, Gene Expression, Western Blot, Mass Spectrometry, Stable Transfection, shRNA